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Location： Home > Phospho Antibodies > CDC2(Phospho-Tyr15) Antibody

CDC2(Phospho-Tyr15) Antibody#11244

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Product Detail

Product NameCDC2(Phospho-Tyr15) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from Human CDC2.

Target NameCDC2

ConjugateUnconjugated

Other NamesCDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;

Accession NoSwiss-Prot: P06493
NCBI Protein: NP_001163877.1

Uniprot P06493

Gene ID 983;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 34kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) Antibody #11244.
Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #11244.
Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #11244.

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Y Gu, et al. (1992) EMBO J. 11(11): 3995

If you have published an article using product 11244, please notify us so that we can cite your literature.

et al,Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents. , (2012),
PMID: 22686412
et al,SUMOylation-regulated Protein Phosphorylation, Evidence from Quantitative Phosphoproteomics Analyses. , (2013),
PMID: 21685386
et al,Adaptive Gene Regulation of Pyruvate Dehydrogenase Kinase Isoenzyme 4 in Hepatotoxic Chemical-Induced Liver Injury and Its Stimulatory Potential for DNA Repair and Cell Proliferation.In J Recept Signal Transduct Res on 2011 Feb by Minori Dateki, Megumi Kunitomo,et al..PMID:21182459 , (2011),
PMID: 21182459
et al,SUMOylation-regulated protein phosphorylation, evidence from quantitative phosphoproteomics analyses.In J Biol Chem on 2011 Aug 5 by Qi Yao, Hui Li,et al..PMID:21685386 , (2011),
PMID: 21685386
et al,Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents. In Biochem J on 2012 Sep 15 by Philip M Kubara, Sophie Kern茅is-Golsteyn, et al..PMID: 22686412 , (2012),
PMID: 22686412
et al,Prolonged Mitotic Arrest Induced by Wee1 Inhibition Sensitizes Breast Cancer Cells to Paclitaxel.In Oncotarget on 2017 May 13 by Cody W Lewis , Zhigang Jin,et al..PMID: 29088738 , (2017),
PMID: 29088738
et al,Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.In Antioxid Redox Signal on 2017 Jul 10 by Xiao-Jing Shi , Lina Ding,et al..PMID: 27650197 , (2017),
PMID: 27650197
More
et al,Jaridonin-induced G2/M Phase Arrest in Human Esophageal Cancer Cells Is Caused by Reactive Oxygen Species-Dependent Cdc2-tyr15 Phosphorylation via ATM-Chk1/2-Cdc25C Pathway .In Toxicol Appl Pharmacol on 2015 Jan 15 by Yong-Cheng Ma , Nan Su et al..PMID:25450480 , (2015),
PMID: 25450480
et al,Critical reanalysis of the methods that discriminate the activity of CDK2 from CDK1.In Cell Cycle on 2016 May 2 by Nandini Sakurikar , Alan Eastman et al..PMID: 26986210 , (2016),
PMID: 26986210
et al,Cytotoxic amounts of cisplatin induce either checkpoint adaptation or apoptosis in a concentration鈥恉ependent manner in cancer cells. In Biol Cell on 2016 May by Lucy H Swift, Roy M Golsteyn锛宔t al..PMID:26871414 , (2016),
PMID: 26871414
et al,Isolation of a natural product with anti-mitotic activity from a toxic Canadian prairie plant , (2021),
PMID: 34095597

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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