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p44/42 MAP Kinase(Phospho-Thr202) Antibody#11245

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Product Detail

Product Namep44/42 MAP Kinase(Phospho-Thr202) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at threonine 202.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from Human p44/42 MAP Kinase.

Target Namep44/42 MAP Kinase

ConjugateUnconjugated

Other NamesERK; ERT2; Extracellular signal- regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1

Accession NoSwiss-Prot: P27361
NCBI Protein: NP_001035145.1

Uniprot P27361

Gene ID 5595;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 42 44 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase(Phospho-Thr202) Antibody #11245.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202) Antibody #11245 (left) or the same antibody preincubated with blocking peptide #51245 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202) Antibody #11245.

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1

TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
 

If you have published an article using product 11245, please notify us so that we can cite your literature.

et al,Phos-tag SDS-PAGE Systems for Phosphorylation Profiling of Proteins With a Wide Range of Molecular Masses Under Neutral pH Conditions. In Proteomics on 2012 Jan by Eiji Kinoshita, Emiko Kinoshita-Kikuta, et al..PMID: 22121028 , (2012),
PMID: 22121028
et al,MWCNTs induce ROS generation, ERK phosphorylation, and SOD-2 expression in human mesothelial cells.In Int J Toxicol on Jan-Feb 2016 by Min Yu, Riping Chen et al ..PMID: 26111538 , (2016),
PMID: 26111538
et al,Apoptosis-inducing activity of synthetic hydrocarbon-stapled peptides in H358 cancer cells expressing KRASG12C. In Acta Pharm Sin B on 2021 Sep by Cuicui Li, Ni Zhao,et al..PMID:34589388 , (2021),
PMID: 34589388

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;