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NFkB-p105(Phospho-Ser932) Antibody#11251

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Product Detail

Product NameNFkB-p105(Phospho-Ser932) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of NFkB-p105 only when phosphorylated at serine 932.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 932 (E-T-S(p)-F-R) derived from Human NFkB-p105.

Target NameNFkB-p105

ConjugateUnconjugated

Other NamesDNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50

Accession NoSwiss-Prot: P19838
NCBI Protein: NP_001158884.1

Uniprot P19838

Gene ID 4790;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 120 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Western blot analysis of extracts from 293 cells untreated or treated with TNF-a using NFkB-p105(Phospho-Ser932) Antibody #11251.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p105(Phospho-Ser932) Antibody #11251(left) or the same antibody preincubated with blocking peptide(right).

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Soren Beinke et al. (2004) Biochem J. 382(Pt 2): 393

If you have published an article using product 11251, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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