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P38 MAPK(Phospho-Thr180) Antibody#11252

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Product Detail

Product NameP38 MAPK(Phospho-Thr180) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of P38 MAPK only when phosphorylated at Threonine 180.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 180 (E-M-T(p)-G-Y ) derived from Human P38MAPK.

Target NameP38 MAPK

ConjugateUnconjugated

Other NamesMAPK2; MAPKAPK-2; MAPKAPK2

Accession NoSwiss-Prot: Q16539
NCBI Protein: NP _001306.1

Uniprot Q16539

Gene ID 1432;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT29 cells untreated or treated with UV using P38 MAPK(Phospho-Thr180) Antibody #11252.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK(Phospho-Thr180) Antibody #11252(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from HL60 cells, treated with TNFa or calf intestinal phosphatase (CIP), using P38 MAPK (Phospho-Thr180) Antibody #11252.

Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

Kim JE, et al. (2005) J Proteome Res Jul-Aug; 4(4): 1339-1346
Meng F, et al. (2005) Am J Physiol Cell Physiol May 25
Jin ZH, et al. (2005)Oncogene Mar 17; 24(12): 1973-1981

If you have published an article using product 11252, please notify us so that we can cite your literature.

et al,Lidocaine Attenuates Proinflammatory Cytokine Production Induced by Extracellular Adenosine Triphosphate in Cultured Rat Microglia. , (2010),
PMID: 20686009
et al,A solute carrier family 22 member 3 variant rs3088442 G锟?A associated with coronary heart disease inhibits lipopolysaccharide-induced inflammatory response. In J Biol Chem on 2015 Feb 27 by Lu Li, Meian He,et al..PMID:25561729 , (2015),
PMID: 25561729
et al,Nephrotoxicity induced by cisplatin is primarily due to the activation of the 5-hydroxytryptamine degradation system in proximal renal tubules , (2021),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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