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P38 MAPK(Phospho-Tyr182) Antibody#11253

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Product Detail

Product NameP38 MAPK(Phospho-Tyr182) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB;IHC;IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of P38MAPK only when phosphorylated at tyrosine 182.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from Human P38 MAPK.

Target NameP38 MAPK

ConjugateUnconjugated

Other NamesMAPK2; MAPKAPK-2; MAPKAPK2

Accession NoSwiss-Prot: Q16539
NCBI Protein: NP _001306.1

Uniprot Q16539

Gene ID 1432;

Calculated MW38kD

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IHC 1:100 - 1:300.  IF 1:50-200

Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK(Phospho-Tyr182) Antibody #11253.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK(Phospho-Tyr182) Antibody #11253(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP), using P38 MAPK (Phospho-Tyr182) Antibody #11253.

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d

If you have published an article using product 11253, please notify us so that we can cite your literature.

et al,Disruption of xCT inhibits cancer cell metastasis via the caveolin-1/娄脗-catenin pathway. , (2008),
PMID: 19015640
et al,SilibInIn A decreases statIn?Induced PCSK9 expression In human hepatoblastoma HepG2 cells. In Mol Med Rep on 2019 Aug by Dong Z, Zhang W,et al..PMID:31173243 , (2019),
PMID: 31173243
et al,Amiloride alleviates morphine tolerance by suppressing ASIC3-dependent neuroinflammation in the spinal cord. In Eur J Pharmacol on 2024 Jan 15 by Liba Gei, Yan Yan,et al..PMID:37918499 , (2024),
PMID: 37918499
et al,Nephrotoxicity induced by cisplatin is primarily due to the activation of the 5-hydroxytryptamine degradation system in proximal renal tubules , (2021),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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