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Tau(Phospho-Thr212) Antibody#11257

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Product Detail

Product NameTau(Phospho-Thr212) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at threonine 212.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 212 (S-R-T(p)-P-S) derived from Human Tau.

Target NameTau

ConjugateUnconjugated

Other NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau

Accession NoSwiss-Prot: P10636
NCBI Protein: NP _001116538.1

Uniprot P10636

Gene ID 4137;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Thr212) Antibody #11257(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Gong CX, et al. (2006) J Biomed Biotechnol. 2006: 31825
Zheng-Fischhofer Q, et al. (1998) Eur J Biochem. 252:542

If you have published an article using product 11257, please notify us so that we can cite your literature.

et al,Extra-virgin olive oil attenuates amyloid-尾 and tau pathologies in the brains of TgSwDI mice. In J Nutr Biochem on 2015 Dec by Hisham Qosa, Loqman A Mohamed,et al..PMID:26344778 , (2015),
PMID: 26344778
et al,TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ , (2023),
PMID: 37357416

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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