Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > Connexin 43 (phospho-Ser368) Antibody

Connexin 43 (phospho-Ser368) Antibody#11258

Availability: Yes
Total Amount:$
Product Detail

Product NameConnexin 43 (phospho-Ser368) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Connexin43 only when phosphorylated at serine 368.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 368 (R-A-S(p)-S-R) derived from Human Connexin 43.

Target NameConnexin 43

ConjugateUnconjugated

Other NamesCX43; CXA1; CXN-43; GJA1;

Accession NoSwiss-Prot: P17302
NCBI Protein: NP_000156.1

Uniprot P17302

Gene ID 2697;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 (Phospho-Ser368) Antibody #11258 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 293 cells untreated or treated with PMA using Connexin 43 (phospho-Ser368) Antibody #11258.

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211
Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353,
Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654
W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444
 

If you have published an article using product 11258, please notify us so that we can cite your literature.

et al,A novel role of SIRT2 in regulating gap junction communications via connexin-43 in bovine cumulus-oocyte complexes.InJ Cell Physiol on 2020 Feb 10. by Xu D, He H et al..PMID:32039484 , (2020),
PMID: 32039484
et al,Electric stimulus opens intercellular spaces in skin.In J Biol Chem.On 2014 Jan 24 by Hama S, Kimura Y et al..PMID:24318878 , (2014),
PMID: 24318878

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;