Product Detail
Product NameEGFR(phospho-Tyr1110) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1110.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1110 (P-V-Y(p)-H-N) derived from Human EGFR.
Target NameEGFR
ConjugateUnconjugated
Other NamesERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
Gene ID
1956;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(phospho-Tyr1110) Antibody #11264.
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Kaisa Erjala, et,al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111.
Judit Anido, et,al. (2003) Clin. Cancer Res ; 9: 1274.
Julian Andreev, et,al. (2001) J. Biol. Chem ; 276: 20130 - 20135.
Suil Kim, et,al. (2002) Am J Physiol Lung Cell Mol Physiol, ; 283: 67.
If you have published an article using product 11264, please notify us so that we can cite your literature.
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