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MARCKS(Phospho-Ser162) Antibody#11265

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Product Detail

Product NameMARCKS(Phospho-Ser162) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from Human MARCKS.

Target NameMARCKS

ConjugateUnconjugated

Other NamesMACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate

Accession NoSwiss-Prot: P29966
NCBI Protein: NP_002347.5

Uniprot P29966

Gene ID 4082;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with PMA using MARCKS(Phospho-Ser162) Antibody #11265.
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser162) Antibody #11265.
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

If you have published an article using product 11265, please notify us so that we can cite your literature.

et al,Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia. In Cell Transplant on Jan-Dec 2021 by Guangming Zhang, Zelin Lai, et al..PMID:34470528 , (2021),
PMID: 34470528
et al,Phosphoproteome of signaling by ErbB2 in ovarian cancer cells. In Biochim Biophys Acta Proteins Proteom on 2022 Apr 1 by C Sidhanth, S Bindhya,et al..PMID:35158093 , (2022),
PMID: 35158093

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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