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Merlin(Phospho-Ser518) Antibody#11266

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Product Detail

Product NameMerlin(Phospho-Ser518) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Merlin only when phosphorylated at serine 518.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin.

Target NameMerlin

ConjugateUnconjugated

Other NamesMERL; NF2; Neurofibromin 2; SCH; Schwannomerlin

Accession NoSwiss-Prot: P35240
NCBI Protein: NP_000259.1

Uniprot P35240

Gene ID 4771;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 69kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) using Merlin(Phospho-Ser518) Antibody #11266.
Immunofluorescence staining of methanol-fixed Hela cells using Merlin(Phospho-Ser518) Antibody #11266.
Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) Antibody #11266.

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.

Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394.
Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715.
R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545.
Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.

If you have published an article using product 11266, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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