Product NameHistone H2A.X(Phospho-Ser139) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.

Target NameHistone H2A.X

ConjugateUnconjugated

Other NamesH2A.X; H2AFX; H2a/x; HIST5-2AX;

Accession NoSwiss-Prot: P16104
NCBI Protein: NP_002096.1

Uniprot P16104

Gene ID 3014;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 15kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200