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CREB(Phospho-Ser129) Antibody#11273

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Product Detail

Product NameCREB(Phospho-Ser129) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CREB only when phosphorylated at serine 129.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 129 (I-L-S(p)-R-R) derived from Human CREB.

Target NameCREB

ConjugateUnconjugated

Other NamesCREB-1; CREB1;

Accession NoSwiss-Prot: P16220
NCBI Protein: NP _004370.1

Uniprot P16220

Gene ID 1385;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with UV(lane 2) using CREB(Phospho-Ser129) Antibody #11273.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser129) Antibody #11273(left) or the same antibody preincubated with blocking peptide(right).
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948.
Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543.
Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.
Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.

If you have published an article using product 11273, please notify us so that we can cite your literature.

et al,IcariIn reduces high glucose?Induced endothelial progenitor cell dysfunction via InhibitIng the p38/CREB pathway and activatIng the Akt/eNOS/NO pathway. In Exp Ther Med on 2019 Dec by Chen S, Wang Z, et al..PMID:31772646 , (2019),
PMID: 31772646
et al,Small Molecule Inhibitors Targeting Gα i 2 Protein Attenuate Migration of Cancer Cells. In Cancers (Basel) on 2020 Jun 19 by Silvia Caggia, Subhasish Tapadar, et al..PMID: 32575572 , (2020),
PMID: 32575572
et al,Up-regulation of astrocyte excitatory amino acid transporter 2 alleviates central sensitization in a rat model of chronic migraine. In J Neurochem on 2020 Nov by Xue Zhou, Jie Liang, et al..PMID:31872442 , (2020),
PMID: 31872442
et al,Saikosaponin a alleviates depressive-like behavior by regulating the BDNF-TrkB-CREB signaling pathway in a mouse model of chronic unpredictable mild stress , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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