Product Detail
Product Namep62Dok(phospho-Tyr398) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of p62Dok only when phosphorylated at tyrosine 398.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 398 (E-G-Y(p)-E-L) derived from Human p62Dok.
Target Namep62Dok
ConjugateUnconjugated
Other NamesDOK1
Accession NoSwiss-Prot: Q99704
NCBI Protein: NP_001372.1
Uniprot
Q99704
Gene ID
1796;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 62kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using p62Dok(phospho-Tyr398) Antibody #11277.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok(Phospho-Tyr398) Antibody #11277(left) or the same antibody preincubated with blocking peptide(right).
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Michael J. Wick, et al. (2001) J. Biol. Chem ; 276: 42843 - 42850.
Paul D. Simoncic, et al. (2006) Mol. Cell. Biol ; 26: 4149 - 4160.
Nadia Dub
If you have published an article using product 11277, please notify us so that we can cite your literature.
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