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PKR(Phospho-Thr446) Antibody#11280

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Product Detail

Product NamePKR(Phospho-Thr446) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of PKR only when phosphorylated at threonine 446.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.

Target NamePKR

ConjugateUnconjugated

Other NamesADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR

Accession NoSwiss-Prot: P19525
NCBI Protein: NP_001129123.1

Uniprot P19525

Gene ID 5610;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. 

Western blot analysis of lysates from NIH/3T3 cells treated with IFN 2500U/ml 30', using PKR (Phospho-Thr446) Antibody. The lane on the right is blocked with the phospho peptide.

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Abujiang Pataer, et,al. (2002) Cancer Res; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 - 18745.
 

If you have published an article using product 11280, please notify us so that we can cite your literature.

et al,Airborne Particulate Matter Selectively Activates Endoplasmic Reticulum Stress Response in the Lung and Liver Tissues. , (2010),
PMID: 20554909
et al,Airborne particulate matter selectively activates endoplasmic reticulum stress response in the lung and liver tissues. In Am J Physiol Cell Physiol on 2010 Oct by Suzette Laing, Guohui Wang, et al..PMID: 20554909 , (2010),
PMID: 20554909
et al,Fluoxetine Inhibits NLRP3 Inflammasome Activation: Implication in Depression.In Int J Neuropsychopharmacol.On 2016 Sep 21 by Du RH, Tan J et al..PMID:27207922 , (2016),
PMID: 27207922
et al,Inhibition of the Integrated stress response by Epstein-Barr virus oncoprotein LMP1 attenuates epithelial cell differentiation and lytic viral reactivation. , (2025),
PMID: 39951426

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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