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p70 S6 Kinase(Phospho-Ser424) Antibody#11284

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Product Detail

Product Namep70 S6 Kinase(Phospho-Ser424) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 424.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 424 (P-V-S(p)-P-V) derived from Human p70 S6 Kinase.

Target Namep70 S6 Kinase

ConjugateUnconjugated

Other NamesKS6B1; P70-S6K; RPS6KB1; S6K;

Accession NoSwiss-Prot: P23443
NCBI Protein: NP_003152.1

Uniprot P23443

Gene ID 6198;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 70 85 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser424) Antibody #11284.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser424) Antibody #11284(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, centrosomal and nuclear staining using p70 S6 Kinase(Phospho-Ser424) Antibody #11284.
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484

If you have published an article using product 11284, please notify us so that we can cite your literature.

et al,Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma锟?In Cancer on 2011 Aug 15 by Dan Liu, Yi Huang, et al..PMID:21387259 , (2011),
PMID: 21387259
et al,Dauricine Inhibits Insulin-Like Growth factor-I-induced Hypoxia Inducible Factor 1alpha Protein Accumulation and Vascular Endothelial Growth Factor Expression in Human Breast Cancer Cells.In Acta Pharmacol Sin on 2009 May by Xu-dong Tang , Xin Zhou,et al..PMID:19349962 , (2009),
PMID: 19349962
et al,Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest.In Mol Med Rep on 2016 May by Bin Fan , Fu-Qaing Li et al..PMID: 27035647 , (2016),
PMID: 27035647
et al,Predictive role of SLC1A5 in neuroblastoma prognosis and immunotherapy. , (2025),
PMID: 39875895

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;