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BIM(Phospho-Ser69) Antibody#11288

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Product Detail

Product NameBIM(Phospho-Ser69) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of BIM only when phosphorylated at serine 69.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 69 (P-A-S(p)-P-G) derived from Human BIM.

Target NameBIM

ConjugateUnconjugated

Other NamesBCL2-like protein 11; BCL2L11; BOD; Bcl-2 related ovarian death protein;

Accession NoSwiss-Prot: O43521
NCBI Protein: NP_006529.1

Uniprot O43521

Gene ID 10018;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 23kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM(Phospho-Ser69) Antibody #11288(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using BIM(Phospho-Ser69) Antibody #11288.
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-a1, isoform Bim-a2 and isoform Bim-a3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis

Fukazawa H, et al. (2004) Mol Cancer Ther ; 3(10): 1281-1288
Luciano F, et al. (2003) Oncogene ; 22(43): 6785-6793

If you have published an article using product 11288, please notify us so that we can cite your literature.

et al,miR-137 and miR-197 induce apoptosis and suppress tumorigenicity by targeting MCL-1 in multiple myeloma.In Clin Cancer Res on 2015 May 15 by Yijun Yang , Fei Li et al..PMID:25724519 , (2015),
PMID: 25724519

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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