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Gab1(Phospho-Tyr627) Antibody#11291

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Product Detail

Product NameGab1(Phospho-Tyr627) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of Gab1 only when phosphorylated at tyrosine 627.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.

Target NameGab1

ConjugateUnconjugated

Other NamesGrowth factor receptor bound protein 2-associated protein 1

Accession NoSwiss-Prot: Q13480
NCBI Protein: NP_002030.2

Uniprot Q13480

Gene ID 2549;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Gab1(Phospho-Tyr627) Antibody #11291.
Immunofluorescence staining of methanol-fixed Hela cells using Gab1(Phospho-Tyr627) Antibody #11291.
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904.
Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307.
Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.

If you have published an article using product 11291, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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