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CREB(Phospho-Ser142) Antibody#11300

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Product Detail

Product NameCREB(Phospho-Ser142) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CREB only when phosphorylated at serine142.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 142(D-L-S(p)-S-D) derived from Human CREB.

Target NameCREB

ConjugateUnconjugated

Other NamesCREB

Accession NoSwiss-Prot: P16220; NCBI Gene ID: 1385; NCBI mRNA: NM_004379.3; NCBI Protein: NP_004370.1

Uniprot P16220

Gene ID 1385;

Sdspage MW43KD

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details
 Predicted MW: 43kd
 Immunohistochemistry: 1:50~1:100 
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser142) Antibody (#11300).
Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using CREB(Phospho-Ser142) Antibody #11300.

 Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

If you have published an article using product 11300, please notify us so that we can cite your literature.

et al,Gene expression pattern in PC12 cells with reduced PMCA2 or PMCA3 isoform: selective up-regulation of calmodulin and neuromodulin , (2012),
PMID: 21912933
et al,Methamphetamine Activates Trace Amine Associated Receptor 1 to Regulate Astrocyte Excitatory Amino Acid Transporter-2 via Differential CREB Phosphorylation During HIV-Associated Neurocognitive Disorders. In Front Neurol on 2020 Nov 25 by Irma E Cisneros, Anuja Ghorpade, et al..PMID:33324330 , (2020),
PMID: 33324330

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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