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Location： Home > Phospho Antibodies > GSK3α/β(Phospho-Tyr279/216) Antibody

GSK3α/β(Phospho-Tyr279/216) Antibody#11301

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Product Detail

Product NameGSK3α/β(Phospho-Tyr279/216) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB, IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of GSK3α/β only when phosphorylated at tyrosine 279/216.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3α/β.

Target NameGSK3α/β

ConjugateUnconjugated

Other NamesFactor A; GSK-3 alpha/beta; kinase GSK3-alpha/beta

Accession NoSwiss-Prot:P49840
Gene ID:2931

Uniprot P49840

Gene ID 2931;

Concentration1.0mg/ml

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:1000), IHC 1:100 - 1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α/β(Phospho-Tyr279/216) Antibody #11301 (left) or the same antibody preincubated with blocking peptide #51301 (right).
Immunofluorescence staining of methanol-fixed Hela cells using GSK3α/β(Phospho-Tyr279/216) Antibody #11301.
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3α/β(Phospho-Tyr279/216) Antibody #11301.
Immunofluorescence staining of methanol-fixed MEF cells using GSK3α/β(Phospho-Tyr279/216) Antibody #11301.
Western blot analysis of extracts of various cell lines,using GSK3α/β(Phospho-Tyr279/216) Antibody#11301.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of GSK3 alpha/beta (Phospho-Y279/216) expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.

glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011],

If you have published an article using product 11301, please notify us so that we can cite your literature.

et al,Association of cellular localization of glycogen synthase kinase 3beta in the digestive tract with cancer development. , (2009),
PMID: 19639192
et al,Amelioration of neurodegenerative changes in cellular and rat models of diabetes-related Alzheimer隆炉s disease by exendin-4 , (2012),
PMID: 21901364
et al,Amelioration of neurodegenerative changes in cellular and rat models of diabetes-related Alzheimer's disease by exendin-4. In Age (Dordr) on 2012 Oct by Song Chen, Ai-ran Liu, et al..PMID:21901364 , (2012),
PMID: 21901364
et al,Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. In Toxicol Lett on 2013 Mar 27 by Hongxia Zhang, Junjie Hou, et al..PMID: 23353032 , (2013),
PMID: 23353032
et al,Activation of GSK3尾 by Sirt2 is required for early lineage commitment of mouse embryonic stem cell. In PLoS One on 2013 Oct 18 by Xiaoxing Si, Wen Chen, et al..PMID: 24204656 , (2013),
PMID: 24204656
et al,Herpes Simplex Virus type-1 infection induces synaptic dysfunction in cultured cortical neurons via GSK-3 activation and intraneuronal amyloid-尾 protein accumulation.In Sci Rep on 2015 Oct 21 by Roberto Piacentini, Domenica Donatella Li Puma et al ..PMID:26487282 , (2015),
PMID: 26487282
et al,Ca2+-dependent release of ATP from astrocytes affects herpes simplex virus type 1 infection of neurons. In Glia on 2021 Jan by Domenica Donatella Li Puma, Maria Elena Marcocci,et al..PMID:32818313 , (2021),
PMID: 32818313

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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