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IkB-b(Phospho-Ser23) Antibody#11304

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Product Detail

Product NameIkB-b(Phospho-Ser23) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of IkB-b only when phosphorylated at serine 23.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b.

Target NameIkB-b

ConjugateUnconjugated

Other NamesI-kappa-B-beta; IKB-B; IKBB; NF-kappa-BIB; NF-kappaB inhibitor beta

Accession NoSwiss-Prot: Q15653
NCBI Protein: NP_001001716.1

Uniprot Q15653

Gene ID 4793;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 48kd
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-b(Phospho-Ser23) Antibody #11304(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from HT29 cells, treated with CA or calf intestinal phosphatase (CIP), using IκB-β (Phospho-Ser23) Antibody #11304.

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174.
DiDonato J, et al. (1996) Mol Cell Biol 16(4): 1295-304

If you have published an article using product 11304, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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