Product Detail
Product NameKeratin 18(Phospho-Ser33) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from Human Keratin 18 (CK18).
Target NameKeratin 18
ConjugateUnconjugated
Other NamesCK 18; CK18; CYK18; Cytokeratin endo B; K18
Accession NoSwiss-Prot: P05783
NCBI Protein: NP_000215.1
Uniprot
P05783
Gene ID
3875;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Keratin 18(Phospho-Ser33) Antibody #11306.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Phospho-Ser33) Antibody #11306(left) or the same antibody preincubated with blocking peptide(right).
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
Moll, R. et al. (1982) Cell 31, 11-24.
If you have published an article using product 11306, please notify us so that we can cite your literature.
Yes
