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SHP-1(Phospho-Tyr536) Antibody#11318

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Product Detail

Product NameSHP-1(Phospho-Tyr536) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB, IHC, IF, ELISA

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of SHP-1 only when phosphorylated at tyrosine 536.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1.

Target NameSHP-1

ConjugateUnconjugated

Other Names70Z-SHP; HCP; HCPH; Hematopoietic cell protein-tyrosine phosphatase; PTN6

Accession NoSwiss-Prot: P29350
NCBI Protein: NP_002822.2

Uniprot P29350

Gene ID 5777;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
WB 1:500-1:2000;
IHC 1:100-1:300;
ELISA 1:10000; IF 1:50-200
Western Blot analysis of RAW264.7+EGF cells
Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 5'. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma. The picture on the right is blocked with the phospho peptide.

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.
Kanagasundaram V, et al. (1999) Mol Cell Biol; 19(6): 4079-4092.
Hauck CR, et al. (1999) Infect Immun; 67(10): 5490-5494.

If you have published an article using product 11318, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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