Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Smad2(Phospho-Ser467) Antibody

Smad2(Phospho-Ser467) Antibody#11322

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(10) Other
Product Detail

Product NameSmad2(Phospho-Ser467) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 467 (C-S-S-M-S(p)) derived from Human Smad2.

Target NameSmad2

ConjugateUnconjugated

Other NamesJV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2

Accession NoSwiss-Prot: Q15796
NCBI Protein: NP _001003652.1

Uniprot Q15796

Gene ID 4087;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
IF 1:50-200
IHC 1:50-1:200

Western blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2(Phospho-Ser467) Antibody #11322.

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

If you have published an article using product 11322, please notify us so that we can cite your literature.

et al,Induction of Thoracic Aortic Remodeling by Endothelial-Specific Deletion of MicroRNA-21 in Mice. , (2013),
PMID: 23527070
et al,Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice. In PLoS One on 2013 by Xing-Yi Zhang, Bao-Rong Shen, et al..PMID: 23527070 , (2013),
PMID: 23527070
et al,TGF尾1 promotes gemcitabine resistance through regulating the LncRNA-LET/NF90/miR-145 signaling axis in bladder cancer.In Theranostics on 2017 Jul 22 by Junlong Zhuang, Lan Shen,et al..PMID: 28839463 , (2017),
PMID: 28839463
et al,Inhibition of TGF尾 signaling decreases osteogenic differentiation of fibrodysplasia ossificans progressiva fibroblasts in a novel in vitro model of the disease.In Bone.On 2016 Mar by Micha D, Voermans E et al..PMID:26769004 , (2016),
PMID: 26769004
et al,High glucose increases Cdk5 activity in podocytes via transforming growth factor-尾1 signaling pathway.In Exp Cell Res on 2014 Aug 15 by Yue Zhang, Hongbo Li et al..PMID: 24768698 , (2014),
PMID: 24768698
et al,TGF尾1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT.In Sci Rep on 2015 Jul 8 by Junlong Zhuang , Qun Lu et al..PMID:26152796 , (2015),
PMID: 26152796
et al,Methotrexate-induced epithelial鈥搈esenchymal transition in the alveolar epithelial cell line A549.In Lung on 2016 Dec by Masashi Kawami, Rika Harabayashi et al..PMID: 27604426 , (2016),
PMID: 27604426
More
et al,The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain.In Int J Biochem Cell Biol on 2014 Apr by Qing-Ping Yao, Ping Zhang et al..PMID: 24495875 , (2014),
PMID: 24495875
et al,SMAD2 Mutations Are Associated with Arterial Aneurysms and Dissections. In Hum Mutat on 2015 Dec by Dimitra Micha, Dong-Chuan Guo,et al..PMID:26247899 , (2015),
PMID: 26247899
et al,The Chinese medicine Fufang Zhenzhu Tiaozhi capsule protects against atherosclerosis by suppressing EndMT via modulating Akt1/β-catenin signaling pathway In J Ethnopharmacol on 2022 Jul 15 by Hongtao Diao, Jiawen Cheng, et al..PMID:35447198 , (2022),
PMID: 35447198

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors