Product NameSmad2(Phospho-Thr220) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB, IHC, IF/IC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2.

Target NameSmad2

ConjugateUnconjugated

Other NamesJV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2

Accession NoSwiss-Prot: Q15796
NCBI Protein: NP _001003652.1

Uniprot Q15796

Gene ID 4087;

Concentration1.0mg/ml

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

WB 1:500 - 1:1000, IHC 1:50 - 1:200), IF/IC 1:100 - 1:500