Product NameSmad3(Phospho-Ser425) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.

Target NameSmad3

ConjugateUnconjugated

Other NamesJV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3

Accession NoSwiss-Prot: P84022
NCBI Protein: NP_001138574.1

Uniprot P84022

Gene ID 4088;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 52kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100