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TrkA(Phospho-Tyr791) Antibody#11326

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Product Detail

Product NameTrkA(Phospho-Tyr791) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of TrkA only when phosphorylated at tyrosine 791.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine791 (P-V-Y(p)-L-D) derived from Human TrkA.

Target NameTrkA

ConjugateUnconjugated

Other NamesHigh affinity nerve growth factor receptor precursor; NTRK1; Slow nerve growth factor receptor; TRK; TRK1 transforming tyrosine kinase protein

Accession NoSwiss-Prot: P04629
NCBI Protein: NP_001007793.1

Uniprot P04629

Gene ID 4914;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 140kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Rat and Mouse brain tissue using TrkA(Phospho-Ser791) Antibody #11326.
Immunofluorescence staining of methanol-fixed Hela cells using TrkA(Phospho-Ser791) Antibody #11326.

Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.

Wiese S, et al. Proc Natl Acad Sci U S A. 2007 Oct 23; 104(43):17210-5.
Valdez G, et al. Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12270-5
Inoue K, et al. J Biol Chem. 2007 Aug 17;282(33):24175-84

If you have published an article using product 11326, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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