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TrkB(Phospho-Tyr515) Antibody#11327

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Product Detail

Product NameTrkB(Phospho-Tyr515) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 515.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 515 (P-Q-Y(p)-F-G) derived from Human TrkB.

Target NameTrkB

ConjugateUnconjugated

Other NamesBDNF/NT-3 growth factors receptor precursor; GP145-TrkB; GP145-TrkB/GP95-TrkB; NTRK2; Trk-B

Accession NoSwiss-Prot: Q16620
NCBI Protein: NP_001007098.1

Uniprot Q16620

Gene ID 4915;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 140kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using TrkB(Phospho-Tyr515) Antibody #11327.
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Woronowicz A, et al. Glycobiology. 2007 Jan;17(1):10-24.
Mojsilovic-Petrovic J, et al. J Neurosci. 2006 Sep 6;26(36):9250-63.
Lewis MA, et al. Mol Pharmacol. 2006 Apr;69(4):1396-404.
Cai D, et al. Physiol Genomics. 2006 Feb 14;24(3):191-7.

If you have published an article using product 11327, please notify us so that we can cite your literature.

et al,Sex-specific and region-specific changes in BDNF篓CTrkB signalling in the hippocampus of 5-HT1A receptor and BDNF single and double mutant mice. , (2012),
PMID: 22464183
et al,The novel squamosamide derivative FLZ enhances BDNF/TrkB/CREB signaling and inhibits neuronal apoptosis in APP/PS1 mice.In Acta Pharmacol Sin on 2010 Mar by Li N, Liu GT et al..PMID:20154710 , (2010),
PMID: 20154710
et al,Lactobacillus pentosus LPQ1 attenuates depressive-like behavior in BALB/C mice induced by chronic unpredictable mild stress (CUMS) , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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