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Location： Home > Phospho Antibodies > a-catenin(Phospho-Ser641) Antibody

a-catenin(Phospho-Ser641) Antibody#11330

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Product Namea-catenin(Phospho-Ser641) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of a-catenin only when phosphorylated at serine 641.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 641 (D-D-S(p)-D-F) derived from Human a-catenin.

Target Namea-catenin

ConjugateUnconjugated

Other NamesCadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen

Accession NoSwiss-Prot: P35221
NCBI Protein: NP_001894.2

Uniprot P35221

Gene ID 1495;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 100kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela cells untreated or treated with starvation using a-catenin(Phospho-Ser641) antibody #11330.
Western blot analysis of extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), using α-catenin (Phospho-Ser641) Antibody #11330.

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.

Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765
Drees, F. et al. (2005) Cell 123, 903-915.
Yamada, S. et al. (2005) Cell 123, 889-901.
Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.

If you have published an article using product 11330, please notify us so that we can cite your literature.

et al,EGF-induced ERK activation promotes CK2-mediated disassociation of alpha-Catenin from beta-Catenin and transactivation of beta-Catenin. , (2009),
PMID: 19941816
et al,Force-dependent allostery of the α-catenin actin-binding domain controls adherens junction dynamics and functions.In Nat Commun. On 2018 Nov 30 by Ishiyama N, Sarpal R et al..PMID: 30504777 , (2018),
PMID: 30504777
et al,2?Aminothiazole Derivatives as Selective Allosteric Modulators of the Protein Kinase CK2. 2. Structure-Based Optimization and Investigation of Effects Specific to the Allosteric Mode of Action.In J Med Chem.On 2019 Feb 28 by Bestgen B, Kufareva I et al..PMID:30689946 , (2019),
PMID: 30689946
et al,Ginsenoside Rh2 activates α-catenin phosphorylation to inhibit lung cancer cell proliferation and invasion.In Exp Ther Med on 2020 Apr; by Zhang G, He L, et al..PMID: 32256776 , (2020),
PMID: 32256776
et al,EGF-induced ERK activation promotes CK2-mediated disassociation of alpha-Catenin from beta-Catenin and transactivation of beta-Catenin. In Mol Cell on 2009 Nov 25 by Ji H, Wang J,et al..PMID:19941816 , (2009),
PMID: 19941816
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by Weiwei Yang, Yan Xia, et al..PMID: 23123196 , (2012),
PMID: 23123196
et al,Helicobacter pylori Induces Cell Migration and Invasion Through Casein Kinase 2 in Gastric Epithelial Cells.In Helicobacter on 2014 Dec by Yeo Song Lee, Do Yeon Lee et al..PMID: 25052887 , (2014),
PMID: 25052887
More
et al,α-catenin phosphorylation is elevated during mitosis to resist apical rounding and epithelial barrier leak. In bioRxiv [Preprint] on 2024 Sep 6 by Phuong M Le, Jeanne M Quinn,et al..PMID:39282345 , (2024),
PMID: 39282345
et al,α-catenin phosphorylation is elevated during mitosis to resist apical rounding and epithelial barrier leak. , (2025),
PMID: 39782767

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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