Product NameAKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.

Target NameAKT1/AKT2/AKT3

ConjugateUnconjugated

Other Names RAC-PK-alpha; Protein kinase B;

Accession NoSwiss-Prot: P31749 P31751 Q9Y243
NCBI Protein: NP_001014431.1 NP_001617.1 NP_005456.1

Uniprot P31749

Gene ID 207;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200