Product Detail
Product Namecofilin1(phospho-Tyr139) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsIHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin.
Target Namecofilin1
ConjugateUnconjugated
Other NamesCFL; CFL1;
Accession NoSwiss-Prot: P23528
NCBI Protein: NP_005498.1
Uniprot
P23528
Gene ID
1072;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 19kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1(Phospho-Tyr139) Antibody #11506(left) or the same antibody preincubated with blocking peptide(right).
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
Arber, S. et al. (1998) Nature 393, 805-809.
Yang, N. et al. (1998) Nature 393, 809-812.
If you have published an article using product 11506, please notify us so that we can cite your literature.
Yes
