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Her3/ErbB3(phospho-Tyr1328) Antibody#11510

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Product Detail

Product NameHer3/ErbB3(phospho-Tyr1328) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Her3/ErbB3 only when phosphorylated at tyrosine 1328.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3.

Target NameHer3/ErbB3

ConjugateUnconjugated

Other Names Tyrosine kinase-type cell surface receptor HER3

Accession NoSwiss-Prot: P21860
NCBI Protein: NP_001005915.1

Uniprot P21860

Gene ID 2065;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from A431 cells untreated or treated with EGF using Her3/ErbB3(phospho-Tyr1328) Antibody #11510.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3(Phospho-Tyr1328) Antibody #11510(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using Her3/ErbB3(phospho-Tyr1328) Antibody #11510.
Binds and is activated by neuregulins and NTAK.

Holbro, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8933-8938.
Kobayashi, M. et al. (2003) Oncogene 22, 1294-1301.
Kim, H.H. et al. (1994) J. Biol. Chem. 269, 24747-24755.
Yarden, Y. and Sliwkowski, M.X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137.

If you have published an article using product 11510, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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