Product NameeIF2a(phospho-Ser49) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of eIF2a only when phosphorylated at Serine 49.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a.

Target NameeIF2a

ConjugateUnconjugated

Other NamesEukaryotic translation initiation factor 2 subunit alpha

Accession NoSwiss-Prot: P05198
NCBI Protein: NP_004085.1

Uniprot P05198

Gene ID 1965;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200