Product Detail
Product NameeIF4B(phospho-Ser422) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.
Target NameeIF4B
ConjugateUnconjugated
Accession NoSwiss-Prot: P23588
NCBI Protein: NP_001408.2
Uniprot
P23588
Gene ID
1975;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B(phospho-Ser422) Antibody #11513.
Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B(Phospho-Ser422) Antibody #11513(left) or the same antibody preincubated with blocking peptide(right).
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
If you have published an article using product 11513, please notify us so that we can cite your literature.
et al,Mammalian target of rapamycin complex 1 (mTORC1) and 2 (mTORC2) control the dendritic arbor morphology of hippocampal neurons. In J Biol Chem on 2012 Aug 31 by Malgorzata Urbanska, Agata Gozdz, et al..PMID: 22810227
, (2012),
PMID:
22810227
Yes
