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PLCg1(phospho-Tyr771) Antibody#11523

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Product Detail

Product NamePLCg1(phospho-Tyr771) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC-g1.

Target NamePLCg1

ConjugateUnconjugated

Other NamesPhosphoinositide phospholipase C; Phospholipase C-gamma-1;

Accession NoSwiss-Prot: P19174
NCBI Protein: NP_002651.2

Uniprot P19174

Gene ID 5335;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody #11523.
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) Antibody #11523.
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
Margolis, B. et al. (1989) Cell 57, 1101-1107.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.

If you have published an article using product 11523, please notify us so that we can cite your literature.

et al,Discovery of a Novel Src Homology-2 Domain Containing Protein Tyrosine Phosphatase-2 (SHP2) and Cyclin-Dependent Kinase 4 (CDK4) Dual Inhibitor for the Treatment of Triple-Negative Breast Cancer , (2022),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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