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ABL1/2 (phospho-Tyr393/439) Antibody#11530

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Product Detail

Product NameABL1/2 (phospho-Tyr393/439) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of ABL1/2 only when phosphorylated at tyrosine393/439.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 393/439 (D-T-Y(p)-T-A) derived from Human ABL1/2.

Target NameABL1/2

ConjugateUnconjugated

Other NamesAbelson murine leukemia viral oncogene homolog 1; c-ABL; p150

Accession NoSwiss-Prot:P00519
Gene ID:25

Uniprot P00519

Gene ID 25;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 210kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) Antibody #11530(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) Antibody #11530.
Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) Antibody #11530 and the same antibody preincubated with blocking peptide.

Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
Pluk, H. et al. (2002) Cell 108, 247-259.

If you have published an article using product 11530, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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