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VEGFR2(phospho-Tyr1059) Antibody#11531

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Product Detail

Product NameVEGFR2(phospho-Tyr1059) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of VEGFR2 only when phosphorylated at Tyrosine 1059.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1059 (P-D-Y(p)-V-R)derived from Human VEGFR2.

Target NameVEGFR2

ConjugateUnconjugated

Other NamesFLK1; CD309; VEGFR; KDR;

Accession NoSwiss-Prot: P35968
NCBI Protein: NP_002244.1

Uniprot P35968

Gene ID 3791;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 230kd
Western blotting: 1:1000
Western blot analysis of extracts from HT29 cells untreated or treated with SCF-1 using VEGFR2(phospho-Tyr1059) Antibody #11531.
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions

Zeng H, et al. (2001) J Biol Chem. 276(35): 32714-32719.
Dougher M, et al. (1999) Oncogene. 18(8): 1619-1627.

If you have published an article using product 11531, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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