Product Detail
Product NamePaxillin(phospho-Tyr88) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 88.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.
Target NamePaxillin
ConjugateUnconjugated
Other NamesPAXI; PXN;
Accession NoSwiss-Prot: P49023
NCBI Protein: NP_001074324.1
Uniprot
P49023
Gene ID
5829;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody #11538.
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody #11538.
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
If you have published an article using product 11538, please notify us so that we can cite your literature.
Yes
