Product Detail
Product NameCyclin E1(phospho-Thr395) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
Target NameCyclin E1
ConjugateUnconjugated
Other NamesCCNE; CCNE1;
Accession NoSwiss-Prot: P24864
NCBI Protein: NP_001229.1
Uniprot
P24864
Gene ID
898;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 48kd
Western blotting: 1:1000
Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1(phospho-Thr395) Antibody #11541.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
Won K.A., Reed S.I.EMBO J. 15:4182-4193(1996)
Welcker M., Singer J., Loeb K.R., Grim J., Bloecher A., Mol. Cell 12:381-392(2003)
If you have published an article using product 11541, please notify us so that we can cite your literature.
Yes
