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EGFR(phospho-Tyr1197) Goat Polyclonal Antibody#11551

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Product Detail

Product NameEGFR(phospho-Tyr1197) Goat Polyclonal Antibody

Host SpeciesGoat

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing goat with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total EGFR protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y(p)-L-R) derived from Human EGFR.

Target NameEGFR

ConjugateUnconjugated

Other Names Receptor tyrosine-protein kinase ErbB-1; ERBB1; kinase EGFR

Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2

Uniprot P00533

Gene ID 1956;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from PMA-treated HUVEC cells or EGF-treated MDA cells using EGFR(phospho-Tyr1197) goat polyclonal antibody #11551.
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia

Corbalan-Garcia S, et al. (1996) Mol Cell Biolt; 16(10): 5674-5682
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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