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Cdc25B(Phospho-Ser149) Antibody#11553

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Product Detail

Product NameCdc25B(Phospho-Ser149) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityMs

SpecificityThe antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.

Target NameCdc25B

ConjugateUnconjugated

Other NamesCDC25B

Accession NoSwiss-Prot:P30306
Gene ID:12531

Uniprot P30306

Gene ID 12531;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 62kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody #11553.
Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody #11553.

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Li G, et al. (2010) Retrovirology.7:59.
Wang Z,et al. (2010) BMC Cancer.10:233.
Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.

If you have published an article using product 11553, please notify us so that we can cite your literature.

et al,Ser149 Is Another Potential PKA Phosphorylation Target of Cdc25B in G2/M Transition of Fertilized Mouse Eggs. , (2011),
PMID: 21212267
et al,Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs. In J Biol Chem on 2011 Mar 25 by Jianying Xiao, Chao Liu, et al..PMID: 21212267 , (2011),
PMID: 21212267

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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