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CK2a(phospho-Thr360/Ser362) Antibody#11572

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Product Detail

Product NameCK2a(phospho-Thr360/Ser362) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of CK2 only when phosphorylated at thronine 360.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2a.

Target NameCK2a

ConjugateUnconjugated

Other NamesCKII; CK2A1; CSNK2A1

Accession NoSwiss-Prot: P68400
NCBI Protein: NP_001894.2

Uniprot P68400

Gene ID 1457;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 42kd
Western blotting: 1:1000

Western blot of CK2a(Phospho- Thr360/Ser362) antibody(#11572) and CK2a antibody(#21572) in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2a(Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2a and CK2a when Ser362 was mutated to alanine .

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The a and a' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001)
Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002)
Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005)
Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000)

If you have published an article using product 11572, please notify us so that we can cite your literature.

et al,Phenelzine, a small organic compound mimicking the functions of cell adhesion molecule L1, promotes functional recovery after mouse spinal cord injury.In Restor Neurol Neurosci. On 2018 July 22 by Li R, Sahu S et al..PMID: 29889084 , (2018),
PMID: 29889084
et al,The intracellular domain of L1CAM binds to casein kinase 2伪 and is neuroprotective via inhibition of the tumor suppressors PTEN and p53.In J Neurochem on 2015 Jun by Yan Wang , Melitta Schachner et al..PMID: 25727698 , (2015),
PMID: 25727698
et al,Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice. In Dis Model Mech on 2017 Sep 1 by Junping Xu, Chengliang Hu, et al..PMID: 28714852 , (2017),
PMID: 28714852

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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