Product Detail
Product Nameβ-catenin(phospho-Tyr333) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of b-catenin only when phosphorylated at tyrosine 333.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 333 (Y-T-Y(p)-E-K) derived from Human b-catenin
Target Nameβ-catenin
ConjugateUnconjugated
Other NamesCTNNB1; CATNB; CTNB1; CTNNB;
Accession NoSwiss-Prot:Q16539
Gene ID:1432
Uniprot
Q16539
Gene ID
1432;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML.
Lillehoj E.P., Lu W., Kiser T., Goldblum S.E., Kim K.C.Biochim. Biophys. Acta 1773:1028-1038(2007)
Weiske J., Albring K.F., Huber O.Proc. Natl. Acad. Sci. U.S.A. 104:20344-20349(2007)
Bahmanyar S., Kaplan D.D., Deluca J.G., Giddings T.H. Jr., O'Toole E.T., Winey M., Salmon E.D., Casey P.J., Nelson W.J., Barth A.I.Genes Dev. 22:91-105(2008)
If you have published an article using product 11574, please notify us so that we can cite your literature.
et al,Selective GPR55 Antagonism Reduces Chemoresistance in Cancer Cells .In Pharmacol Res on 2016 Sep by Nagendra S Singh , Michel Bernier et al..PMID: 27423937
, (2016),
PMID:
27423937
et al,Disintegration of the Cav-1/β-catenin complex protects neuronal death by promoting β-catenin nuclear translocation after ischemia-reperfusion injury
, (2022),
PMID:
Yes
