Product Detail
Product NameHistone H3(Phospho-Thr11) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Histone H3 only
when phosphorylated at threonine 11.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of threonine 11(K-S-T(p)-G-G) derived from Human Histone H3.
Target NameHistone H3
ConjugateUnconjugated
Other NamesH3/a H3/m H3.3A; H3/c H3/o H3F3B; H3/d; H3/f; H3/h
Accession NoSwiss-Prot:P68431
Gene ID:8350
Uniprot
P68431
Gene ID
8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 17kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from U87 cells untreated or treated with EGF using Histone H3(Phospho-Thr11) Antibody #11577.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Preuss U., Landsberg G., Scheidtmann K.H.
Nucleic Acids Res. 31:878-885(2003)
Shimada M., Niida H., Zineldeen D.H., Tagami H., Tanaka M., Saito H., Nakanishi M.Cell 132:221-232(2008)
Metzger E., Yin N., Wissmann M., Kunowska N., Fischer K., Friedrichs N., Patnaik D., Higgins J.M., Potier N., Scheidtmann K.H., Buettner R., Schule R.Nat. Cell Biol. 10:53-60(2008)
If you have published an article using product 11577, please notify us so that we can cite your literature.
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803
, (2012),
PMID:
22901803
et al,RNF8 Mediates Histone H3 Ubiquitylation and Promotes Glycolysis and Tumorigenesis.In J Exp Med on 2017 Jun 5 by Yan Xia , Weiwei Yang,et al..PMID: 28507061
, (2017),
PMID:
28507061
Yes
