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Niban(Phospho-Ser602) Antibody#11578

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Product Detail

Product NameNiban(Phospho-Ser602) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Niban only when phosphorylated at serine 602.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 602 (R-A-S(p)-A-I) derived from Human Niban.

Target NameNiban

ConjugateUnconjugated

Other NamesFAM129A

Accession NoSwiss-Prot: Q9BZQ8
NCBI Protein: NP_443198.1

Uniprot Q9BZQ8

Gene ID 116496;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 150kd
Western blotting: 1:500~1:1000

Western blot analysis in vitro kinase assays were performed by mixing purified bacterially expressed WT His–Niban or the His–Niban S602A with or without purified active AKT1 using Niban (Phospho-Ser602) Antibody #11578.

Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response

Vill

If you have published an article using product 11578, please notify us so that we can cite your literature.

et al,AKT-dependent phosphorylation of Niban regulates nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis. , (2012),
PMID: 22510990
et al,AKT鈥恉ependent phosphorylation of Niban regulates nucleophosmin鈥恆nd MDM2鈥恗ediated p53 stability and cell apoptosis. In EMBO Rep on 2012 Jun 1 by Haitao Ji, Zhiyong Ding, et al..PMID: 22510990 , (2012),
PMID: 22510990
et al,P2X7R Antagonism After Subfailure Overstretch Injury of Blood Vessels Reverses Vasomotor Dysfunction and Prevents Apoptosis.In Purinergic Signal on 2017 Dec by Weifeng Luo, Daniel Feldman,et al..PMID: 28905300 , (2017),
PMID: 28905300

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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