Product NamePHB(Phospho-Thr258) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of PHB only when phosphorylated at threonine 258.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from Human PHB.

Target NamePHB

ConjugateUnconjugated

Other NamesProhibitin; PHB1

Accession NoSwiss-Prot: P35232;
NCBI Gene ID: 5245;
NCBI Protein: NP_001268425.1.

Uniprot P35232

Gene ID 5245;

Sdspage MW29

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

 Western blotting: 1:500~1:1000