Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > beta-catenin (Phospho-Ser715) Antibody

beta-catenin (Phospho-Ser715) Antibody#11594

Availability: Yes
Total Amount:$
Product Detail

Product Namebeta-catenin (Phospho-Ser715) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of beta-catenin only when phosphorylated at serine 715.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 715 (D-P-S(p)-Y-R) derived from Human beta-catenin.

Target Namebeta-catenin

ConjugateUnconjugated

Other NamesCatenin beta-1; CTNB1; CTNNB; CTNNB1

Accession NoSwiss-Prot: P35222
NCBI Gene ID: 1499

Uniprot P35222

Gene ID 1499;

Sdspage MW92kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Target SpeciesHuman

Application Details

Western blotting: 1:500~1:1000

Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human primary glioblastoma multiforme (GBM) specimens using beta-catenin (Phospho-Ser715) Antibody #11594.
Western blot analysis of extract from U87 cells transfected with either PKCd siRNA targeting or control siRNA were treated withWnt3a (100 ng ml 1) for 8 h. WB was performed with nuclearlysates of the cells with the beta-catenin (Phospho-Ser715) Antibody #11594.

 Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML

 

If you have published an article using product 11594, please notify us so that we can cite your literature.

et al,Tumour suppressor TRIM33 targets nuclear b-catenin degradation , (2015),
PMID: 25639486
et al,Tumour suppressor TRIM33 targets nuclear 尾-catenin degradation.In Nat Commun on 2015 Feb 2 by Jianfei Xue, Yaohui Chen et al..PMID:25639486 , (2015),
PMID: 25639486

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;