Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > PDHK1(Phospho-Thr338) Antibody

PDHK1(Phospho-Thr338) Antibody#11596

Datasheet PDF
Availability： The lead time is currently 1 week.
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(9) Other
Product Detail

Product NamePDHK1(Phospho-Thr338) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of PDHK1 only when phosphorylated at threonine 338.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 338(Y-S-T(p)-A-P) derived from Human PDHK1.

Target NamePDHK1

ConjugateUnconjugated

Other NamesPDK1; PDH kinase 1

Accession NoSwiss-Prot: Q15118
NCBI Protein: NP_001265478.1

Uniprot Q15118

Gene ID 5163;

Calculated MW49kd

Concentration1.0mg/mL

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Target SpeciesHuman

Application Details

 Western blotting: 1:500~1:1000

Immunoblotting analysis of PDHK1 and PDH phosphorylation in mitochondria. Mitochondrial fractions were prepared and immunoblotted with indicated antibodies
 Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
 
Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M.J. Biol. Chem. 270:28989-28994(1995)            
The MGC Project TeamGenome Res. 14:2121-2127(2004)
 

 

If you have published an article using product 11596, please notify us so that we can cite your literature.

et al,Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. , (2016 Mar),
PMID: 26942675
et al,Associations of PGK1 promoter hypomethylation and PGK1-mediated PDHK1 phosphorylation with cancer stage and prognosis: a TCGA pan-cancer analysis. In Cancer Commun (Lond) on 2019 Oct 2 by Shao F, Yang X,et al..PMID:31578148 , (2019),
PMID: 31578148
et al,O-GlcNAcylation of PGK1 coordinates glycolysis and TCA cycle to promote tumor growth.In Nat Commun on 2020 Jan 7; by Nie H, Ju H, et al..PMID:31911580 , (2020),
PMID: 31911580
et al,Identification of a novel non-ATP-competitive protein kinase inhibitor of PGK1 from marine nature products. In Biochem Pharmacol on 2021 Jan by Yuying Wang, Lulu Sun, et al..PMID:33212041 , (2021),
PMID: 33212041
et al,Suppression of Pyruvate Dehydrogenase Kinase by Dichloroacetate in Cancer and Skeletal Muscle Cells Is Isoform Specific and Partially Independent of HIF-1伪. In Int J Mol Sci on 2021 Aug 10 by Nives 艩korja Mili膰, Klemen Dolinar, et al..PMID:34445316 , (2021),
PMID: 34445316
et al,Inhibition of phosphoglycerate kinase 1 attenuates autoimmune myocarditis by reprogramming CD4+ T cell metabolismInCardiovasc ResOn2023 Jun 13byYang Lu?1?2?3?4,?Ning Zhao et al..PMID: 36726197 , (2023),
PMID: 36726197
et al,Integrative proteogenomic characterization of early esophageal cancerInNat CommunOn 2023 Mar 25byLingling Li , Dongxian Jiang et al..PMID:36966136 , (2023),
PMID: 36966136
More
et al,PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis. In Cell Death Dis on 2024 Feb 24 by Hao Liu, Xintian Chen, et al..PMID:38402202 , (2024),
PMID: 38402202
et al,Hypoxia-induced downregulation of PGK1crotonylation promotes tumorigenesis bycoordinating glycolysis and the TCA cycle. In Nat Commun on 2024 Aug 12 by Zihao Guo, Yang Zhang,et al..PMID:39134530 , (2024),
PMID: 39134530

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors