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PDHK1(Phospho-Tyr243) Antibody#11597

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Product Detail

Product NamePDHK1(Phospho-Tyr243) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Rt

SpecificityThe antibody detects endogenous level of PDHK1 only when phosphorylated at tyrosine 243.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 243(D-L-Y(p)-Y-I) derived from Human PDHK1.

Target NamePDHK1

ConjugateUnconjugated

Other NamesPDK1; PDH kinase 1

Accession NoSwiss-Prot: Q15118
NCBI Protein: NP_001265478.1

Uniprot Q15118

Gene ID 5163;

Calculated MW49kd

Concentration1.0mg/mL

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Target SpeciesHuman

Application Details

Western blotting: 1:500~1:1000 

U87 cells were stimulated with or without hypoxia for 6 h. Immunoblotting analyses of mitochondrial lysates were performed with the indicated antibodies
 Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
 
Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M.J. Biol. Chem. 270:28989-28994(1995)
The MGC Project TeamGenome Res. 14:2121-2127(2004)

 

If you have published an article using product 11597, please notify us so that we can cite your literature.

et al,Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. , (2016 Mar),
PMID: 26942675
et al,Pyruvate dehydrogenase inactivation causes glycolytic phenotype in BAP1 mutant uveal melanoma. In Oncogene on 2022 Feb by Anna Han, Vivian Chua,et al..PMID:35046531 , (2022),
PMID: 35046531

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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