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PGK1 (Acetyl-Lys388) Antibody#11599

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Product Detail

Product NamePGK1 (Acetyl-Lys388) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

ApplicationsWB IHC IP

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of PGK1 only when acetylated at Lysine 388.

Immunogen TypePeptide

Immunogen DescPeptide sequence around acetylation site of lysine 388(E-D-K(Acetyl)-V-S) derived from Human PGK1.

ConjugateUnconjugated

Other NamesMGC117307,MGC142128,MGC8947,MIG10,PGKA

Accession NoSwiss-Prot#: P00558
NCBI Gene ID: 5230
NCBI mRNA#: NM_000291.3
NCBI Protein#: NP_000282.1

Uniprot P00558

Gene ID 5230;

Calculated MW45KD

Concentration1.0mg/mL

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details

 WB dilution: 1:500~1:1000

 IHC dilution: 1:50~1:100

 IP dilution: 1:20~1:50

The indicated His-PGK1 proteins immobilized on Ni-NTA agarose beads was incubated with Flag-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, in the presence of [γ-32P] ATP. Autoradiography was performed.

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).

If you have published an article using product 11599, please notify us so that we can cite your literature.

et al,Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy.In Mol Cell on 2017 Mar 2 by Xu Qian , Xinjian Li,et al..PMID: 28238651 , (2017),
PMID: 28238651
et al,Identification of a novel non-ATP-competitive protein kinase inhibitor of PGK1 from marine nature products. In Biochem Pharmacol on 2021 Jan by Yuying Wang, Lulu Sun, et al..PMID:33212041 , (2021),
PMID: 33212041

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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