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PKA α/β CAT (Phospho-Thr197) Antibody#11667

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Product Detail

Product NamePKA α/β CAT (Phospho-Thr197) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA α/β CAT.

Target NamePKA α/β CAT

ConjugateUnconjugated

Other NamesPRKACA ; PRKACB; PKACA;

Accession NoSwiss-Prot#: P17612;
NCBI Gene#: 5566;
NCBI Protein#: NP_002721.1.

Uniprot P17612

Gene ID 5566;

Sdspage MW40kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from mouse brain cells using PKA α/β CAT (Phospho-Thr197) Antibody #11667.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody #11667 (left)or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed A549 cells using PKA α/β CAT (Phospho-Thr197) Antibody #11667.

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

If you have published an article using product 11667, please notify us so that we can cite your literature.

et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms. , (2025),
PMID: 39736347

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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